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Test ID: IH Calcitonin
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Calcitonin
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Immunohistochemistry Stain
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Useful For
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Identification of calcitonin-producing C-cells, from which medullary thyroid carcinoma (MTC) originates.
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Method name and description
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- Polyclonal Rabbit Anti-Human Calcitonin.
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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Calcitonin (thyrocalcitonin) is a 32 amino acid linear polypeptide hormone that participates in calcium and phosphorus metabolism. Immunohistochemical staining with anti-calcitonin antibody is an effective way of demonstrating calcitonin-producing parafollicular C cells in the thyroid. Immunohistochemical studies of C-cell hyperplasia and medullary thyroid carcinomas stain will predominately stain positive for calcitonin. Other tissues such as the lungs and intestinal tract can also synthesize calcitonin. Expression of Calcitonin in both non-neoplastic and neoplastic neuroendocrine cells of these organs, such as carcinoids and small cell lung carcinomas, may be seen.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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