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Test ID: IH BCL6
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BCL6
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Immunohistochemistry Stain
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Useful For
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This antibody is useful for classification of diffuse large B-cell lymphomas, follicular lymphomas and Burkitt’s lymphomas.
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Method name and description
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- Monoclonal Mouse Anti-Human BCL6 Protein, Clone PG-B6p
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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The proto-oncogene BCL6 encodes a 92-98 kDa POZ/zinc finger protein. Through its six C-terminal zinc-finger motifs, the BCL6 protein binds to specific DNA sequences of target genes, where it acts as a transcriptional repressor. BCL6 expression may be ubiquitous, as low levels are detected in many tissues. However, high levels of BCL6 expression are limited to cells of the Blymphoid linage and skeletal muscle. BCL6 may be implicated in chromosomal translocations where the regulatory region of the BCL6 gene is replaced by a heterologous reciprocal partner such as the immunoglobulin genes. BCL6 rearrangement is one of the most common genetic abnormalities in B-cell non-Hodgkin’s lymphoma, with an especially high frequency (28-40%) in diffuse large cell lymphoma.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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