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Test ID: Heinz Body Stain
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Heinz Body Stain
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Useful For
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Evaluation of hemolytic anemias with RBC enzyme deficiencies (e.g.G6PD deficiency)
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Method name and description
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Supravital staining and microscopic examination of stained slide.
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Clinical information
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Heinz bodies are insoluble intracellular inclusions of denatured Hemoglobin resulting from oxidative injury to the red cells leading to denaturation and precipitation of haemoglobins, particularly abnormal or unstable haemoglobins and haemoglobin in erythrocytes with glucose-6-phosphate dehydrogenase deficiency. These bodies are attached to the membrane of the erythrocyte resulting in destruction of the red cell in the reticuloendothelial system leading to hemolytic anemia.
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Specimen type / Specimen volume / Specimen container
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Whole blood sample (may be part of the CBC)
Container/Tube: EDTA.
Specimen Volume: 1 ml.
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Collection instructions / Special Precautions / Timing of collection
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Testing should be started as soon as possible after collection.
Test is unreliable in infants under six months of age.
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Relevant clinical information to be provided
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History of oxidative hemolytic anemia including G6PD deficiency, Medications history
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Storage and transport instructions
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Ambient/Refrigerated (Specimens are stable 48 hours, refrigerated)
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Specimen Rejection Criteria
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Wrong container
Clotted/hemolyzed specimen.
Mislabeled or unlabeled specimens.
Specimen older than 48hrs.
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Biological reference intervals and clinical decision values
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Factors affecting test performance and result interpretation
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Turnaround time / Days and times test performed / Specimen retention time
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1 working day
Test is performed daily
Specimen is retained for 3 Days at 2 - 8 °C
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