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Test ID: Beta-D-Glucan
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Beta-D-Glucan
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Commercially available highly sensitive microplate-based assay that detects 1,3-β-D-Glucan in serum
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Useful For
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Aiding in the diagnosis of invasive fungal infections caused by various fungi, including Aspergillus species, Fusarium species, Candida species, and Pneumocystis jirovecii among immunocompromised and intensive care patients
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Method name and description
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Commercially available protease zymogen-based colorimetric assay
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Clinical information
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Invasive Fungal Infection (IFIs) cause severe illness in the critically ill and immunocompromised patients and are associated with high morbidity and mortality. More aggressive medical care, including immunosuppressive therapy and intensive care has greatly increased the population of patients at risk of IFI.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum
Specimen Volume: 3-10 ml
Specimen container: Red top serum gel tube.
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Relevant clinical information to be provided
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- Relevant clinical history indicating need for the test
- Antifungal therapy
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Storage and transport instructions
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≤ 2 hours at room temperature
> 2 hours store at 2-8 °C. Specimen whould reach the laboratory within 24 hrs of collection
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Specimen Rejection Criteria
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- Contaminated specimen
- Improper container
- Improper transport
- Unlabeled /Mislabeled specimen
- Duplicate specimen received within 72 hrs.
- Infant and pediatric samples
- Samples sent without infectious diseases specialist recommendation or not from intenive care
- Quantity not sufficient
- No relevant clinical details provided with the request. (Please note that where applicable, the relevant clinical details should reflect those on the patient’s electronic record at the time the order was placed).
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Biological reference intervals and clinical decision values
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Negative result
- Beta-D-Glucan Test: Negative
Postive result
- Beta-D-Glucan Test: Positive with Optical density (OD) value
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Factors affecting test performance and result interpretation
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Improper Container
Improper Transport
Hemolysis
Turbid Serum
Evaluted levels of Immunoglobulin G
Please be aware that the test cut-off values have been established exclusively for adults and are not applicable to infants and pediatric patients. Hence, this test should only be performed on adult patients.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time
Depends on day of receipt as samples are only processed on Tuesdays
Days and times test performed
- Batched and test performed every Tuesday at the HGH microbiology laboratory
- Specimens accepted on all days
Specimen retention time
- 48 hrs. after final report
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