Overview:
The Molecular Pathophysiology (MP) Core facility aims to provide molecular biology/signaling pathway analysis related services and support to research investigators from various institutions. It is our goal to offer the highest quality products and services possible in the most cost-effective and timely manner.
Core services and technology:
Our primary services are responsible for providing technology and assays for following assays and protocols:
- Analysis of various signaling pathways such as PI3-kinase/AKT, JAK/STAT, Ubiquitin/proteasome, NF-κB/p65, Map kinases and JNK pathway
- Growth and maintenance of various cell lines
- Cell proliferation assays
- Apoptotic assays
- Protein estimations
- Western blotting analysis for protein expression
- Gene silencing and gene profiling techniques
- Transfection assays
- Luciferase promotor assays
- Colony assays.
- Assessment of Protein post-translational modifications such as phosphorylation and ubiquitination of specific proteins
- Cytochrome c analysis
- Immunoprecipitation and co-immunoprecipitation assays
- Determination of cytokines in cell culture and biological samples
To avail the services of the MP core, the interested researchers must submit the project details to the core facility lead. Once the project details have been reviewed by the Core team it will be forwarded to TRI management for scoring and approval
iBright FL1500 Imaging System
The iBright FL1500 Imaging System supports the main imaging applications of fluorescent, chemiluminescent, and colorimetric western blots, in addition to fluorescent stained nucleic acid gels, fluorescent stained protein gels, colorimetric stained protein gels, and colorimetric membrane stains.
Agilent xCELLigence Real-Time Cell Analysis (RTCA) DP (Dual Purpose)
The Agilent RTCA DP instrument continuously measures cell invasion and migration (CIM) and monitors cell health, behavior, and function using label-free cellular impedance.
The three cradles of the DP instrument enable three separate electronic 16-well plates to be controlled and monitored in parallel or independently of one another. This flexible plate batch processing allows maximum productivity for multiple users. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator.
Team and Contact Details:
Dr. Shahab Uddin Khan, PhD (Head, Molecular Pathophysiology Core Facility)
+974 40253220
skhan34@hamad.qa
MP Core Facility, Hamad Medical City, Building 320, TRI, Doha
Select Publications:
Khan AQ, Mohamed EN, Hakeem I, Nazeer A, Kuttikrishnan S, Prabhu KS, Nawaz Z, Ahmad A. Zayed H, Uddin S. Sanguinarine induces apoptosis in papillary thyroid cancer cells via generation of reactive oxygen species Molecules 25. pii: E1229, 2020.
Prabhu K, Raza A, Karedath T, Raza SS, Ahmed EI, Kuttikrishnan S, Therachiyil L, Kulinski M, Dermine S, Junejo K, Steinhoff M, Uddin S. Non-coding RNAs as regulators and markers of targeting in breast cancer and breast cancer stem cells. Cancer (Basel)12. pii: E351, 2020.
Khan AQ, Ahmed EA, Elareer NR, Prabhu KS, Siveen KS, Kulinski M, Ahmad A, Steinhoff M, Uddin S. Curcumin-mediated apoptotic cell death in papillary thyroid cancer and cancer stem-like cells through targeting of JAK/STAT3 signaling pathway. Int J Mol Sci;21. pii: E438, 2020.
Kuttikrishnan S, Siveen KS, Prabhu KS, Khan AQ, Ahmed EI, Akhtar S, Ali TA, Merhi M, Dermime S, Steinhoff M, Uddin S. Curcumin induces mitochondrial apoptotic cell death via inhibition of PI3-Kinase/AKT pathway in B-precursor acute lymphoblastic leukemia. Front in Oncol ;9: 484, 2019.
Akhtar S, Achkar IW, Siveen KS, Kuttikrishnan S, Prabhu KS, Khan AQ, Ahmed EI, Sahir F, Jerobin J, Raza A, Merhi M, Elsabah HM, Taha R, Omri HE, Zayed H, Dermime S, Steinhoff M, Uddin S. Sanguinarine induces apoptosis pathway in multiple myeloma cell lines via inhibition of the JaK2/STAT3 signaling. Frontiers Oncol; 9:285, 2019.
Collaborating Institutes:
Dermatology, NCCCR, Qatar University, Sidra Medicine, HGH
