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Test ID: IH HBME1
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Mesothelial Cell
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Immunohistochemistry Stain
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Useful For
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Aid in the diagnosis of mesotheliomas. Also good in papillary thyroid carcinoma.
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Method name and description
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- Monoclonal Anti-Mesothelial Cell, clone HBME-1 (anti-HBME-1)
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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Anti-HBME-1 reacts with an antigen present in the microvillous surface of mesothelioma cells. Anti-HBME-1 has been demonstrated to immunostain the membrane and cytoplasm of normal pleural and peritoneal mesothelial cells and of neoplastic epithelial mesothelioma cells. Although both the membrane and cytoplasm of epithelial mesothelioma cells stain positive with HBME-1, the thick membrane staining pattern was found to be a more diagnostically useful marker of malignant mesothelioma. Other normal and malignant tissues detailed in the Performance characteristics section have also been reported to be immunoreactive with HBME-1.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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