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Test ID: IH Granzyme B
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Granzyme B
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Immunohistochemistry Stain
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Useful For
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Identification of the neoplastic counterparts from human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells.
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Method name and description
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- Monoclonal Mouse Anti-Human Granzyme B, Clone GrB-7
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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Granzyme B, a 29 kDa monomer protein, is a member of a subfamily of serine proteases with chymotrypsin-like specificity. For CTL and NK cells to excert their cytolytic activity, the presence of cytoplasmic secretory granules containing granzymes and perforin are pivotal. After granule exocytosis, granzyme B is believed to enter the target cell through a perforin-derived transmembrane channel to induce DNA fragmentation and apoptosis. A high percentage of activated CTL’s, identified by anti-granzyme B, clone GrB-7, and present in tumour biopsies of patients with nodular sclerosis or mixed cellularity Hodgkin’s disease, has been reported to be a strong indicator for an unfavorable outcome.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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