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Test ID: IH Desmin
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Desmin
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Immunohistochemistry Stain
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Useful For
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Expressed in skeletal muscle, Rhabdomyosarcoma and DSRCT.
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Method name and description
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- Monoclonal Mouse Anti-Human Desmin, Clone D33
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section.
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Clinical information
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Desmin belongs to the class III of intermediate filaments, constituting part of the cytoskeleton, and is the characteristic intermediate filament of all three types of muscle cells (skeletal, cardiac, and smooth muscle). Desmin is a 53 kDa protein encoded by nine exons of a gene located at 2q35. Desmin forms a cytoskeletal network across the muscle fibre bordering at the plasma and nuclear membrane and is particularly localized to the subplasmalemmal region and the Z-band.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide e (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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