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Test ID: Entamoeba histolytica Antibody
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Entamoeba histolytica Antibody
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Useful For
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Semi quantitative determination of specific antibodies to Entamoeba histolytica in human serum
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Method name and description
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Indirect hemagglutination test.
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Reporting name
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Entamoeba histolytica Antibody
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Aliases
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E. Histolytica Antibody, Entamoeba; Amoebic Serology; Amebiasis
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum
Specimen Volume: 3-5 mL
Specimen container/Tube: Serum collected in Yellow top gel tube
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Collection instructions / Special Precautions / Timing of collection
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Collection by venipuncture as per organization guidelines
No special precautions / no Timing of collection
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Storage and transport instructions
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Maintain specimen at 2-8°C
The specimens should be transported to Virology Section in cold box immediately
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Specimen Rejection Criteria
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Quantity of sample not sufficient
lipemic sample
Hemolysed sample
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Biological reference intervals and clinical decision values
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- Titer < 1:80 Negative reaction (probable absence of amoeba tissue infection)
- Titer 1:80 & 1:160 Doubtful reaction (repeat after 2-3 weeks)
- Titer ≥ 1:320 Significant reaction (in favour of visceral amoebiasis)
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Factors affecting test performance and result interpretation
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- The specimen should not show any signs of haemolysis, cloudiness, or contamination.
- Repeated freezing and defrosting should be avoided
- Serum samples with very high antibody concentrations may exhibit the prozone phenomenon, resulting in the disappearance of agglutination at low dilutions, which resolves upon further dilution.
- In all cases, clinical, epidemiological, and laboratory findings should be considered before establishing the final diagnosis.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time: within 7 calendar days
Days and times test performed: Twice a week
Specimen retention time: 2 weeks
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