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Test ID: Echinococcus Antibody
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Echinococcus Antibody
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Useful For
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Semi quantitative determination of specific antibodies to Echinococcus granulosus in human serum.
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Method name and description
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Indirect hemagglutination assay (IHA)
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum
Specimen volume: 3-5 mL
Specimen container: Serum collected in Yellow top gel tubes
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Collection instructions / Special Precautions / Timing of collection
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Collection by venipuncture as per organization guidelines
No special precautions/ No timing of collection
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Storage and transport instructions
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Maintain specimen at 2-8°C
The specimens should be transported to Virology Section in cold box immediately
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Specimen Rejection Criteria
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Quantity of blood not sufficient
Wrong blood container
Container breakage / sample leaking
Lipemic sample
Grossly hemolyzed serum
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Biological reference intervals and clinical decision values
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- Titer < 1:160 Non-significant reaction (probable absence of hydatidosis; repeat after 2-3 weeks)
- Titer = 1:160 Doubtful reaction (repeat after 2-3 weeks)
- Titer ≥ 1:320 Significant reaction (in favour of progressive hydatidosis)
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Factors affecting test performance and result interpretation
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- The specimen should not show any signs of haemolysis, cloudiness, or contamination.
- Repeated freezing and defrosting should be avoided
- Serum samples with very high antibody concentrations may exhibit the prozone phenomenon, resulting in the disappearance of agglutination at low dilutions, which resolves upon further dilution.
In all cases, clinical, epidemiological, and laboratory findings should be considered before establishing the final diagnosis.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time: within 7 calendar days
Days and times test performed: twice a week.
Specimen retention time: 2 week.
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