Test ID: Dengue Virus IgM
|
Dengue Virus IgM Antibody
|
|
Useful For
|
Qualitative determination of IgM class antibodies against Dengue virus in human serum or plasma.
|
Method name and description
|
Enzyme-linked Immunosorbent Assay (ELISA)
|
|
Specimen type / Specimen volume / Specimen container
|
Specimen type: Serum or plasma
Specimen volume: 3-5 mL
Specimen container: Plasma collected in sodium heparin, Serum collected in yellow top gel tubes
|
Collection instructions / Special Precautions / Timing of collection
|
Collection by venipuncture as per organization guidelines
No special precautions / No timing of collection
|
Storage and transport instructions
|
Maintain specimen at 2-8°C
The specimens should be transported to Virology Section in cold box immediately
|
Specimen Rejection Criteria
|
Quantity of blood not sufficient
Wrong blood container
Container breakage / sample leaking
Lipemic sample
Grossly hemolyzed serum
|
|
Biological reference intervals and clinical decision values
|
No reference
Negative / Positive / Equivocal
|
Factors affecting test performance and result interpretation
|
Reactive IgM might suggests recent infection with Dengue virus or cross reaction with another flavivirus or vaccine or non-specific reaction.
|
Turnaround time / Days and times test performed / Specimen retention time
|
Turnaround time: Within 7 days
Days and times test performed: Twice a week.
Specimen retention time: 2 weeks
|
|
|