Lab Guide
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Test ID: Dengue Virus IgM
Dengue Virus IgM Antibody
Useful For

Qualitative determination of IgM class antibodies against Dengue virus in human serum or plasma.

Method name and description

Enzyme-linked Immunosorbent Assay (ELISA)

Reporting name

Dengue Virus IgM

Aliases
Specimen type / Specimen volume / Specimen container

Specimen type: Serum or plasma

Specimen volume: 3-5 mL

Specimen container: Plasma collected in sodium heparin, Serum collected in yellow top gel tubes

Collection instructions / Special Precautions / Timing of collection

Collection by venipuncture as per organization guidelines

No special precautions / No timing of collection

Storage and transport instructions

Maintain specimen at 2-8°C

The specimens should be transported to Virology Section in cold box immediately

Specimen Rejection Criteria

Quantity of blood not sufficient

Wrong blood container

Container breakage / sample leaking

Lipemic sample

Grossly hemolyzed serum

Biological reference intervals and clinical decision values

No reference

Negative / Positive / Equivocal

Factors affecting test performance and result interpretation

Reactive IgM might suggests recent infection with Dengue virus or cross reaction with another flavivirus or vaccine or non-specific reaction.

Turnaround time / Days and times test performed / Specimen retention time

Turnaround time: Within 7 days

Days and times test performed: Twice a week.

Specimen retention time: 2 weeks