|
Test ID: Dengue Virus IgM
|
|
Dengue Virus IgM Antibody
|
|
|
|
Useful For
|
Qualitative determination of IgM class antibodies against Dengue virus in human serum or plasma.
|
|
Method name and description
|
Enzyme-linked Immunosorbent Assay (ELISA)
|
|
|
Specimen type / Specimen volume / Specimen container
|
Specimen type: Serum or plasma
Specimen volume: 3-5 mL
Specimen container: Serum collected in Yellow top gel tube
|
|
Collection instructions / Special Precautions / Timing of collection
|
Collection by venipuncture as per organization guidelines
No special precautions / No timing of collection
|
|
Storage and transport instructions
|
Maintain specimen at 2-8°C
The specimens should be transported to Virology Section in cold box immediately
|
|
Specimen Rejection Criteria
|
Quantity of blood not sufficient
Wrong blood container
Container breakage / sample leaking
Lipemic sample
Grossly hemolyzed serum
|
|
|
Biological reference intervals and clinical decision values
|
- Dengue IgM negative: No evidence of infection
- Dengue IgM positive: Result suggests recent infection with Dengue virus or cross reaction with another flavivirus, or vaccine related and should be interpreted with the clinical presentation.
- Dengue IgM equivocal: An equivocal result neither confirms nor excludes antibody detection and should be reviewed in the light of clinical findings. If dengue fever is clinically suspected and in keeping with recent travel, a follow-up sample in 7 - 10 days is recommended to rule out beginning of infection or non-specific reaction.
|
|
Factors affecting test performance and result interpretation
|
- In immunocompromised patients and newborn serological data have restricted value - (May produce a weak or delayed antibody response that can result in low antibody titers or false-negative ELISA results).
- Bacterial contamination may affect the absorbance value
- Repeated freeze–thaw cycles of the sample may affect the absorbance value
- Interferences with hemolytic, lipemic or icteric samples are not observed up to a concentration of 10 mg/mL hemoglobin, 5 mg/mL triglycerides and 0.5 mg/mL bilirubin.
- Cross reactivity with other flaviviruses cannot be excluded and should be considered.
- A precise diagnosis should take into consideration clinical history symptomatology as well as serological data.
|
|
Turnaround time / Days and times test performed / Specimen retention time
|
Turnaround time: Within 7 calendar days
Days and times test performed: Twice a week.
Specimen retention time: 2 weeks
|
|
|
