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Test ID: Dengue Virus IgG
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Dengue Virus IgG Antibody
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Useful For
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Qualitative determination of IgG class antibodies against Dengue virus in human serum or plasma.
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Method name and description
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Enzyme-linked Immunosorbent Assay (ELISA)
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum or plasma
Specimen volume: 3-5 mL
Specimen container: Serum collected in Yellow top gel tube
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Collection instructions / Special Precautions / Timing of collection
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Collection by venipuncture as per organization guidelines
No special precautions / No timing of collection
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Storage and transport instructions
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Maintain specimen at 2-8°C
The specimens should be transported to Virology Section in cold box immediately
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Specimen Rejection Criteria
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Quantity of blood not sufficient
Wrong blood container
Container breakage / sample leaking
Lipemic sample
Grossly hemolyzed serum
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Biological reference intervals and clinical decision values
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- Dengue IgG negative: The sample contains no antibodies against the pathogen.
- Dengue IgG positive: Result suggests past infection with Dengue virus
- Dengue IgG equivocal: An Equivocal result neither confirms nor excludes antibody detection and should be reviewed in the light of clinical findings.
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Factors affecting test performance and result interpretation
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- In immunocompromised patients and newborn serological data have restricted value - (May produce a weak or delayed antibody response that can result in low antibody titers or false-negative ELISA results).
- Bacterial contamination may affect the absorbance value
- Repeated freeze–thaw cycles of the sample may affect the absorbance value
- Interferences with hemolytic, lipemic or icteric samples are not observed up to a concentration of 10 mg/mL hemoglobin, 5 mg/mL triglycerides and 0.5 mg/mL bilirubin.
- In endemic areas, double infection as well as past infection with other flaviviruses should be considered.
- A precise diagnosis should take into consideration clinical history symptomatology as well as serological data.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time: Within 7 calendar days
Days and times test performed: Twice a week.
Specimen retention time: 2 weeks
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