|
Test ID: RPR Test
|
|
Rapid Plasma Reagin test
|
|
Non-treponemal qualitative and semi-quantitative testing procedure for serologic detection of syphilis in patient serum
|
|
Useful For
|
Non-treponemal assays are useful to help distinguish between treated and untreated syphilis, as this test detects antibodies against lecithin, cholesterol and cardiolipin, which are present in many syphilis patients. The tes is also used for monitoring the progression of disease and treatment response.
(For HMC users only: This test cannot be ordered directly, it will be added by lab if Treponema pallidum Ab Antibody found positive)
|
|
Method name and description
|
|
|
|
|
Specimen type / Specimen volume / Specimen container
|
Specimen type: Serum or plasma
Specimen volume: 3-5 mL
Specimen container: Plasma collected in sodium heparin, Serum collected in Yellow top gel tubes
|
|
Collection instructions / Special Precautions / Timing of collection
|
Collection by venipuncture as per organization guidelines
No special precautions/ No timing of collection
|
|
Storage and transport instructions
|
Maintain specimen at 2-8°C
The specimens should be transported to Virology Section in cold box immediately
|
|
Specimen Rejection Criteria
|
Quantity of blood not sufficient
Wrong blood container
Container breakage / sample leaking
Lipemic sample
Grossly hemolyzed serum
|
|
|
Biological reference intervals and clinical decision values
|
No reference
Reactive / Nonreactive
Reactive with titer (1:1), (1:2), (1:4), (1:8), (1:16)…..etc.
|
|
Factors affecting test performance and result interpretation
|
1-False negative results can occur, because of failure to recognize prozone reaction. Prozone reaction occurs in 1% to 2% of patients with secondary syphilis.
2-False positive reaction is reported in disease such as infectious mononucleosis, leprosy, malaria, lupus erythematous, vaccinia and virus pneumonia.
|
|
Turnaround time / Days and times test performed / Specimen retention time
|
Turnaround time: Within 7 days
Days and times test performed: Daily except Friday and Saturday from 7am to 3pm
Specimen retention time: 2 weeks
|
|
|
