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Test ID: Leukocyte Adhesion Deficiency CD11b/CD18
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Leukocyte Adhesion Deficiency CD11b/CD18
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Useful For
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The CD11b [ APC (D12) ] and CD18 [PE Mouse Anti-Human] from BD Biosciences are used for diagnosis of moderate or severe leukocyte adhesion deficiency (LAD) by assessing presence or absence of the CD11b/CD18 expression.
Leukocyte adhesion deficiency (LAD) syndrome is a rare autosomal recessive disorder characterized by immunodeficiency resulting in recurrent infections, impaired wound healing, delayed umbilical cord separation, persistent leukocytosis, and recurrent soft tissue and oral infections.
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Method name and description
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Method name: Flow Cytometric Immunophenotyping.
Flow cytometric immunophenotyping of peripheral blood is performed to evaluate the presence or absence of the CD11/CD18 complex using monoclonal antibodies directed against the CD11b and CD18 antigens.
Test is done by stain, lysis and wash technique and analyzed by Flow cytometer.
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Reporting name
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Leukocyte adhesion deficiency CD11/CD18
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Clinical information
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Leukocyte adhesion deficiency syndrome type 1 (LAD-1) is an autosomal recessive disorder caused by mutations in the common chain (CD18) of the beta2-integrin family. LAD-1 is clinically characterized by recurrent infections, impaired wound healing, delayed umbilical cord separation, persistent leukocytosis, and recurrent soft tissue and oral infections.
Patients with LAD-1 (and other primary immunodeficiency diseases) are unlikely to remain undiagnosed in adulthood. Consequently, this test should not be typically ordered in adults for LAD-1. However, it may be also used to assess immune competence by determining CD18 and 11b expression.
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Aliases
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LAD (Leukocyte Adhesion Deficiency)
LAD1
CD11b
CD18
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Specimen type / Specimen volume / Specimen container
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Specimen type: A fresh whole blood sample.
Container Tube: EDTA Tube.
Specimen Volume: 3 mL,Whole Blood EDTA, For pediatric 500ul (EDTA microtainer).
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Collection instructions / Special Precautions / Timing of collection
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A fresh control blood, from a normal healthy subject is required (Mother Or Father).
Specimens should be processed within 24 hours of sampling.
Transport Immediately after collection in room temperature.
Routine: Sunday through Thursday, Last samples on Thursday should be collected before 11:00 am.
Date and time of draw is required.
Samples Collected on the weekends will be rejected.
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Relevant clinical information to be provided
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Sufficient clinical information relevant to the requested test should be provided.
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Storage and transport instructions
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The specimen should be sent to the laboratory as soon as possbile after collection.
The sample should be stored at room temperature within 24 hours of blood collection.
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Specimen Rejection Criteria
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Insufficient quantity of blood.
Clotted.
Wrong collection tube or anticoagulant.
Outdated specimens.
Improper sample storage.
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Biological reference intervals and clinical decision values
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| Leukocyte adhesion deficiency (LAD) Interpretation |
| Normal |
Presence of CD18/CD 11b |
| Abnormal |
Absence of CD18/CD 11b |
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Factors affecting test performance and result interpretation
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Improper sample storage.
Centrifuged sample.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time: 24 working Hours.
Days and times test performed: Sunday through Thursday, Last samples on Thursday should be collected before 11:00 am.
For serial monitoring, we recommend that specimen draws be performed at the same time of day.
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