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Test ID: Tissue culture
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Tissue culture
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Microbiological culture of tissues including biopsies and bone samples by conventional culture technique
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Useful For
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Infections of wounds ,tissues and biopsy specimens including bone.
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Method name and description
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Microscopy: Gram Stain
Culture: Aerobic and Anaerobic conventional culture technique
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Clinical information
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A biopsy may be defined as a portion of tissue removed from the body for further examination. Tissue obtained whilst operating is particularly precious as the sampling procedure may not be repeatable. Ideally these specimens should be discussed with the laboratory prior to sampling to ensure that transport and processing are timely, and appropriate tests are performed.
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Aliases
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- Tissue Culture
- Culture Tissue
- C Tissue
- Bone culture
- Bone biopsy
- Biopsy culture
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Specimen type / Specimen volume / Specimen container
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Specimen type
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Aneurysm tissue
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Gingiva
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Mass
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Biopsy
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Graft
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Muscle
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Bone
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Granuloma
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Node
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Cartilage
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Heart Valve
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Nodule
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Cellulitis
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Joint tissue
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Pilonidal Cyst
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Cyst
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Ligament
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Placenta/ products of conception
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Colon Biopsy
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Lymph Node
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Polyp
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Renal Cyst
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Tissue
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Tendon
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Gastric biopsy
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Skin Biopsy
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Lung biopsy
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Prosthetic joint tissue
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Note: Tissue received as whole part for example: whole finger, entire toe, big chunk of tissue is not acceptable
Specimen volume
- The specimen should ideally, be large enough to carry out all microscopy preparations and cultures.
- Minimum specimen size will depend on the number of investigations requested. Numbers and frequency of specimen collection are dependent on clinical condition of patient.
Specimen container
- Sterile leak proof container
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Collection instructions / Special Precautions / Timing of collection
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Collection instructions
- The skin surface and surgical area are disinfected before collection, and the specimen is obtained aseptically by an invasive technique.
- For small samples, add a few drops of sterile saline to keep moist.
- Do not allow tissue to dry out.
Note: The portion of the biopsy specimen submitted for culture should be separated from the portion submitted for histopathology
Special Precautions
Do not place sample in formalin as it is unsuitable for microbiological investigations
Timing of collection
- Prior to antimicrobial therapy where possible
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Relevant clinical information to be provided
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- Antimicrobial therapy
- Clinical condition
- Site of infection
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Storage and transport instructions
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- <2 hrs. at room temperature. Do NOT store
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Specimen Rejection Criteria
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- Specimen sent in formalin
- Tissue received as whole part for example: whole finger, entire toe, big chunk of tissue
- Unlabeled/Mislabeled specimens
- Improper container
- Duplicate specimen within 72 hours
- Quantity not sufficient (only culture performed- no microscopy done)
- No relevant clinical details provided with the request. (Please note that where applicable, the relevant clinical details should reflect those on the patient’s electronic record at the time the order was placed).
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Biological reference intervals and clinical decision values
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Negative Microscopy report
No organisms seen
Positive Microscopy report
Organisms seen reported based on Gram stain result and morphology
Note: All STAT Gram stains called to Physician or Nurse in-charge, if positive.
Negative Culture report
- No growth
- Normal skin flora
- Normal skin flora including coliforms
- Mixed growth of coliforms
Positive Culture report
- Significant organism identified and reported with antimicrobial susceptibilities
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Factors affecting test performance and result interpretation
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- Improper collection
- Delayed transport
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time
Microscopy:
- STAT specimens: 1 hour
- Routine specimens:24 hrs
Note:
- No Gram stain done for rectal and intestinal biopsies e.g. colonic biopsy, duodenal aspirates or bone.
Culture: 2-5 days
Note: Preliminary results are released as early as the presumptive identification is available
Note:
- Duodenal aspirate or biopsy - No culture, for ova and parasite exam only.
- Oesophageal swab / biopsy - for fungal culture only.
Days and times test performed
Specimen retention time
- 7 days after final report
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