1. Samples collected in HMC facilities MUST reach the coagulation laboratory within 1 hour of collection.
2. Samples collected in non-HMC facilities / external clients: MUST reach coagulation laboratory within 1 hour of collection, if not applicable, centrifuge the whole blood and carefully remove the plasma (by pipette not by decanting) and send it in a temperature-controlled environment (18- 25 ºC) within 2 hours of centrifugation. If the transportation of the plasma can’t be within a maximum of 2 hours, prepare platelet-poor plasma as follows:

• Re-centrifuge the plasma again.
• Remove the top portion of plasma leaving approximately 250 ul in the bottom to discard.
• The double-centrifuged plasma should be aliquoted into labeled plastic tubes.
• Freeze immediately at -20 ºC or below
• Specimens must arrive frozen.

1. If patient HCT is >55%, test will not be processed. The ordering Physician will be contacted to reorder the test and call the patient to go to Phlebotomy for recollection. Instruct the patient to inform the phlebotomy   about his high HCT% , the phlebotomy will contact the lab to request a coagulation tube after adjusting amount of anticoagulant.

Note:

Collection of blood for coagulation testing through intravenous lines that have been previously flushed with heparin should be avoided, if possible. If the blood must be drawn through an indwelling catheter, possible heparin contamination and specimen dilution must be considered. When obtaining specimens from indwelling lines that may contain heparin, the line should be flushed with 5 mL of saline, and the first 5 mL of blood or 6-times the line volume (dead space volume of the catheter) be drawn off and not used for coagulation testing. For those specimens collected from a normal saline lock (capped off venous port) twice the dead space volume of the catheter and extension set should be discarded.