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Test ID: Diffuse Large B‐Cell Lymphoma (DLBCL) panel by FISH
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Diffuse Large B‐Cell Lymphoma (DLBCL) panel by FISH
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This test panel identifies a set of particular structural chromosome anomalies commonly associated with Diffuse Large B-cell Lymphoma.
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Method name and description
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Interphase Fluorescence In Situ Hybridization (I-FISH) study.
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Clinical information
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This test panel identifies a set of particular structural chromosome anomalies commonly associated with Diffuse Large B-cell Lymphoma using the desired probes, eg: MYC, IGH/BCL2 and BCL6 for corresponding chromosomes 3q27, 8q24, and t(14;18)(q32;q21).
Routine chromosome examination is also required to be performed on all diagnostic samples and is suggested on all bone marrow specimens to exclude abnormalities not identified by the desired FISH probe(s).
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Specimen type / Specimen volume / Specimen container
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1. Specimen Type: Blood/Leukemic Blood
Container/Tube: Green top (sodium heparin)
Specimen Volume: 2-6 mL (Varies depending on the WBC-Count).
(Adult - 3-6 mL ,Child - 2 mL, Infant - 2 mL)
2 .Specimen Type: Bone marrow
Container/Tube: Bone Marrow Transport Media
Specimen Volume: 1-3 mL (Varies depending on the WBC-Count).
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Storage and transport instructions
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Specimen Rejection Criteria
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Consistent with Diagnostic Genomic Division policies.
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Factors affecting test performance and result interpretation
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Diffuse Large B-Cell Lymphoma (DLBCL) could be a lymphoid neoplasm, especially including large (microscopic) B cells. This FISH panel is outlined to distinguish the most common, and/or prognostically-significant abnormalities in DLBCL,
This may help in discrimination between DLBCL and other lymphomas (Swerdlow et al. 2008), as well as revealing ‘double hit’ lymphomas (Aukema et 2011). FISH studies are valuable adjuncts to total chromosome studies, especially when taking after an abnormal clone, evaluating relapse and progression, or when a sample is insufficient for chromosomal examination.
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Turnaround time / Days and times test performed / Specimen retention time
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