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Test ID: Follicular lymphoma panel by FISH
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Follicular lymphoma panel by FISH
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Follicular lymphoma panel by FISH
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Method name and description
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Interphase Fluorescence In Situ Hybridization (I-FISH) study.
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Clinical information
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This assay is useful to analyze Follicular Lymphoma (>75%) and diffuse large B Cell Lymphomas (25%).
The FISH panel is performed to detect IgH (14q32) and BCL2 (18q21. 3), ruling out translocation between t(14; 18)(q32; q21. 3).
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Specimen type / Specimen volume / Specimen container
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1. Specimen Type: Blood/Leukemic Blood:
Container/Tube: Green top (sodium heparin)
Specimen Volume: 2-6 mL (Varies depending on the WBC-Count).
(Adult - 3-6 mL ,Child - 2 mL, Infant - 2 mL)
2. Specimen Type: Bone marrow:
Container/Tube: Bone Marrow Transport Media
Specimen Volume: 1-3 mL (Varies depending on the WBC-Count).
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Collection instructions / Special Precautions / Timing of collection
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Sunday-Wednesday 7:00-15:00
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Storage and transport instructions
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Specimen Rejection Criteria
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Consistent with Diagnostic Genomic Division policies.
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Factors affecting test performance and result interpretation
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The FISH probes particularly identify the t(14;18)(q32;q21) involving BCL2 and IGH genes. This translocation is observed in over 90% of follicular lymphomas and in fewer (20-30%) of Diffuse huge B cell lymphomas.
It is characterized by the abnormal rearrangement of the BCL 2 proto-oncogene on chromosome 18 with the IGH immunoglobulin gene on chromosome 14, resulting in constitutive overexpression of the BCL2 protein which eventually leads to changes in modified cell death (i.e., apoptosis) and tumor cell proliferation.
Assessment for a t(14;18)(q32;q21) is of use within the diagnostic assessment for follicular lymphoma as well as within the assessment of aggressive B cell lymphomas where Burkitt's Lymphoma (BL), Diffuse Large B Cell Lymphoma (DLBCL), and the intermediate subset are symptomatic contemplations.
In aggressive B Cell lymphoma, a t(14;18)(q32;q21) along with MYC rearrangement act as a bad prognosis against the diagnosis of BL. The presence of t(14;18)(q32;q21) is seen more regularly in DLBCLs of germinal cell form and has been reported to be a poor prognostic marker in some findings.
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Turnaround time / Days and times test performed / Specimen retention time
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