|
Test ID: Mantle Cell Lymphoma Panel by FISH
|
|
Mantle Cell Lymphoma Panel by FISH
|
|
Mantle Cell Lymphoma (MCL) is detected by FISH procedure.
|
|
Method name and description
|
Interphase Fluorescence In Situ Hybridization (I-FISH) study.
|
|
Clinical information
|
The FISH test detects the translocation using target probe for 11;14 which is associated with mantle cell lymphoma and may be found in other hematologic tumors, such as plasma cell leukemia, multiple myeloma and chronic lymphocytic leukemia.
This translocation involves the fusion of the immunoglobulin heavy chain gene (14q32) IGH with the cyclin D1 (11q13) CCND1 gene. The FISH test should be considered parallel with chromosome analysis, when possible.
|
|
|
Specimen type / Specimen volume / Specimen container
|
1. Specimen Type: Blood/Leukemic Blood
Container/Tube: Green top (sodium heparin)
Specimen Volume: 2-6 mL (Varies depending on the WBC-Count).
(Adult - 3-6 mL ,Child - 2 mL, Infant - 2 mL)
2. Specimen Type: Bone marrow
Container/Tube: Bone Marrow Transport Media
Specimen Volume: 1-3 mL (Varies depending on the WBC-Count).
|
|
Collection instructions / Special Precautions / Timing of collection
|
Sunday-Wednesday 7:00 -14:00.
|
|
Storage and transport instructions
|
|
|
|
Specimen Rejection Criteria
|
Consistent with Diagnostic Genomic Division policies.
|
|
|
Factors affecting test performance and result interpretation
|
Mantle-Cell Lymphoma (MCL) features a poor prognosis than other small B-Cell Lymphomas, hence a definitive diagnosis is essential. The t(11;14)(q13;q32) associated with MCL associates portions of CCND1 (11q13) and IGH (14q32), resulting in over-expression of cyclin D1.
Hence we perform FISH a sensitive test to identify t(11;14)(q13;q32) in cells obtained from Bone marrow or leukemic blood samples.
|
|
Turnaround time / Days and times test performed / Specimen retention time
|
|
|
|
|
