Bile acids are formed in the liver from cholesterol, conjugated primarily to glycine and taurine, stored and concentrated in the gallbladder, and secreted into the intestine after the ingestion of a meal. Fasting serum bile acids can be used in the diagnosis and prognosis of liver disease. Levels rise in many liver diseases, for example hepatitis and liver cirrhosis. Abnormal levels in fasting patients or immediately after a meal can be used to detect liver disease and damage, impaired liver function, intestinal dysfunction and perhaps a gall bladder blockage. Their measurement may detect some forms of liver disease earlier than standard liver tests because bile acids levels correspond to liver function, rather than liver damage. Bile acids are measured in cholestasis during pregnancy. Patients always present with symptoms of itchiness. If left untreated cholestasis could lead to still birth.