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Test ID: FSH
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Follicle Stimulating Hormone
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Useful For
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To evaluate fertility issues or function of reproductive organs and pituitary function.
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Method name and description
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Electrochemiluminescence immunoassay (ECLIA): Sandwich principle
Electrochemiluminescence immunoassay (ECLIA) intended for use on the cobas e 801 immunoassay analyzer. Patient specimen, biotinylated monoclonal FSH‑specific antibodies, and a monoclonal FSA‑specific antibody labeled with a ruthenium complex form a sandwich complex. After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve.
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Clinical information
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FSH (follicle stimulating hormone), together with LH (luteinizing hormone), belongs to the gonadotropin family. FSH and LH regulate and stimulate the growth and function of the gonads (ovaries and testes) synergistically. In women FSH, in conjunction with LH, stimulates oestrogen secretion and ovulation. In men, FSH serves to induce spermatogonium development. The determination of FSH in conjunction with LH is utilized for the following indications: congenital diseases with chromosome aberrations, polycystic ovaries (PCO), amenorrhea (causes), and menopausal syndrome. Depressed gonadotropin levels in men occur in azoospermia
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Aliases
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Follicle Stimulating Hormone , FSH
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Sodium citrate plasma and sodium fluoride/potassium oxalate are not recommended (values obtained is lowered).
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Storage and transport instructions
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Storage: 5 days at 20 – 25°C
14 days at 2 – 8°C;
6 months at ‑20 °C (± 5 °C)
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample and heat‑inactivated samples.
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Biological reference intervals and clinical decision values
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Sex
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Age
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Reference interval (IU/L)
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From
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To
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Male
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0 Minutes
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1 Year
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0.1-3.3
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Male
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1 Year
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5 Years
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0.1-1.9
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Male
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5 Years
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10 Years
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0.1-2.3
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Male
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10 Years
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15 Years
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0.6-6.9
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Male
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15 Years
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20 years
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0.7-9.7
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Male
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> 20
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4.6-12.4
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Female
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0 Minutes
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1 Year
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1.2-12.5
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Female
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1 Year
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10 Years
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0.5-6.0
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Female
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10 Years
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15 Years
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0.9-8.9
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Female
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15 Years
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20 Years
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0.7-9.7
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Female: more than 20 years old
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Follicular Phase: 4 – 13 IU/L
Ovulation Phase: 5 – 22 IU/L
Luteal Phase: 2 – 8 IU/L
PMP Phase: 26 – 135 IU/L
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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