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Test ID: Gentamicin
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Gentamicin
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Useful For
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Monitoring therapeutic drug level to ensure adequate dosing and to avoid toxic side effects.
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Method name and description
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Kinetic Interaction of Microparticles in a Solution (KIMS)
Kinetic interaction of microparticles in solution (KIMS) as measured by changes in light transmission on the Roche cobas c-systems. Gentamicin antibody is covalently coupled to microparticles and the drug derivative is linked to a macromolecule. The kinetic interaction of microparticles in solutions is induced by binding of drug‑conjugate to the antibody on the microparticles and is inhibited by the presence of Gentamicin in the sample. A competitive reaction takes place between the drug conjugate and Gentamicin in the serum sample for binding to the Gentamicin antibody on the microparticles. The resulting kinetic interaction of microparticles is indirectly proportional to the amount of drug present in the sample.
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Clinical information
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Gentamicin is an aminoglycoside antibiotic that displays broad spectrum, high potency, anti‑bacterial action for most susceptible organisms. It has been most successfully used in the treatment of serious infections especially those caused by gram‑negative bacilli. In patients with pre‑existing renal damage or those to whom gentamicin has been administered for prolonged periods or in doses above the therapeutic range, hearing impairment and/or nephrotoxicity may develop. Therefore, monitoring of peak and trough gentamicin levels is critical in the prevention of these serious complications with the adjustment of dosage administration as indicated.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Lithium heparin tube
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Collection instructions / Special Precautions / Timing of collection
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The time of blood collection must be indicated.
Trough: Just prior to next dose
Peak: 1-2 hour after the end of infusion
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Storage and transport instructions
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Storage: 1 week at 2 – 8°C;
4 weeks at ‑20 °C
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample and heat‑inactivated samples.
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Biological reference intervals and clinical decision values
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Therapeutic range:
Trough range: < 2.0 ug/mL (mg/L)
Peak range: 5- 10 ug/mL (mg/L)
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
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