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Test ID: Haptoglobin
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Haptoglobin
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Useful For
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It is used primarily to help detect and evaluate hemolytic anemia and to differentiate it from other type of anemia.
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Method name and description
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Immunoturbidimetric assay
mmunoturbidimetric assay performed on the Roche cobas c-systems. Human haptoglobin forms a precipitate with a specific antiserum which is determined turbidimetrically.
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Clinical information
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Haptoglobin binds hemoglobin in a strong haptoglobin‑hemoglobin complex(Hp‑Hb), the hemoglobin resulting from pathologically elevated hemolysis is deposited in hepatocytes. Complex formation and the extremely rapid elimination from circulating blood prevent the occurrence of hemoglobinuria with excess renal loss of iron. A reduction in the level of free haptoglobin is indicative of intravascular hemolysis.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
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Storage and transport instructions
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Storage: 3 months at 15 – 25°C
8 months at 2 – 8°C
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample.
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Biological reference intervals and clinical decision values
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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