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Test ID: LDH
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LDH
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Useful For
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To help detect an acute or chronic condition that cause tissue damage or cellular destruction, to determine prognosis/response to treatment of certain cancers.
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Method name and description
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UV assay (L‑lactate to pyruvate)
UV assay performed on the Roche cobas c-systems. Lactate dehydrogenase catalyzes the conversion of L‑lactate to pyruvate; NAD is reduced to NADH in the process The initial rate of the NADH formation is directly proportional to the catalytic LDH activity. It is determined by photometrically measuring the increase in absorbance.
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Clinical information
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The lactate dehydrogenase (LDH) enzyme is widely distributed in tissue, particularly in the heart, liver, muscles and kidneys. Elevated serum levels of LDH have been observed in a variety of disease states. The highest levels are seen in patients with megaloblastic anemia, disseminated carcinoma and shock. Moderate increases occur in muscular disorders, nephrotic syndrome and cirrhosis. Mild increases in LDH activity have been reported in cases of myocardial or pulmonary infarction, leukemia, hemolytic anemia and non‑viral hepatitis.
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Aliases
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Lactate Dehydrogenase, LDH
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
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Storage and transport instructions
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Storage: 7 days at 15 – 25°C
4 days at 2 – 8°C
6 weeks at ‑20 °C
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample.
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Biological reference intervals and clinical decision values
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| Gender |
Age |
Reference Range (U/L) |
| Female |
0 Days - 1 Month |
145-765 |
| Female |
1 Month - 1 Year |
190-420 |
| Female |
1 Year - 3 Years |
165-395 |
| Female |
3 Year - 6 Years |
135-345 |
| Female |
6 Years - 9 Years |
140-280 |
| Female |
9 Years - 12 Years |
120-260 |
| Female |
12 Years - 15 Years |
100-275 |
| Female |
15 Years - 18 Years |
105-230 |
| Female |
> 18 Years |
135-214 |
| Male |
0 Days - 1 Month |
125-735 |
| Male |
1 Month - 1 Year |
170-450 |
| Male |
1 Year - 6 Years |
155-345 |
| Male |
6 Years - 9 Years |
145-300 |
| Male |
9 Years - 12 Years |
120-325 |
| Male |
12 Years - 15 Years |
120-290 |
| Male |
15 Years - 18 Years |
105-235 |
| Male |
> 18 Years |
135-225 |
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
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