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Test ID: Lipase
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Lipase
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Useful For
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To diagnose and monitor pancreatitis or other pancreatic disease.
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Method name and description
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Enzymatic colorimetric assay
Enzymatic colorimetric assay with 1,2‑O‑dilauryl-rac-glycero-3-glutaricacid‑( 6‑methylresorufin) ester as substrate performed on the Roche cobas c-systems. The chromogenic lipase substrate 1,2‑O‑dilauryl-rac-glycero-3-glutaricacid‑(6‑methylresorufin) ester is cleaved by the catalytic action of alkaline lipase solution to form 1,2‑O‑dilauryl-rac‑glycerol and an unstable intermediate, glutaric acid‑(6‑methylresorufin) ester. This decomposes spontaneously in alkaline solution to form glutaric acid and methylresorufin. Addition of detergent and colipase increases the specificity of the assay for pancreatic lipase. The color intensity of the red dye formed is directly proportional to the lipase activity and can be determined photometrically.
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Clinical information
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In addition to α‑amylase, pancreatic lipases have for many years been undeniably the most important clinical chemistry parameters for the differential diagnosis of diseases of the pancreas. The lipase activity determination has gained increasing international recognition because of its high specificity and rapid response. After acute pancreatitis the lipase activity increases within 4‑8 hours, reaches a peak after 24 hours and decreases after 8‑14 days. However, there is no correlation between the lipase activity determined in serum and the extent of damage to the pancreas.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
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Storage and transport instructions
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Storage: 1 week at 20 – 25°C
1 week at 4 – 8°C;
1 year at -20°C (serum)
2 months at ‑15 to -25°C (plasma)
Transport: 4-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample.
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Biological reference intervals and clinical decision values
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Patient Sex Age Range Range (U/L)
Female/Male 0 Days -1 Month 6 - 55
Female/Male 1 Month - 6 Month 4 - 29
Female/Male 6 Month - 1 Year 4 - 23
Female/Male 1 Y - 3 Y 4 - 31
Female/Male 3 Y - 9 Y 3 - 32
Female/Male 9 Y - 18 Y 4 - 29
Female/Male >18Y 13 - 60 |
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
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