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Test ID: DHEA-S
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Dehydroepiandrosterone Sulfate
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Useful For
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To evaluate adrenal gland function, adjunct in the diagnosis of congenital adrenal hyperplasia and to determine the cause of virilization in girls and women.
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Method name and description
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Electrochemiluminescence immunoassay (ECLIA) : Competition principle
Electrochemiluminescence immunoassay (ECLIA) intended for use on the cobas e 801 immunoassay analyzer. Patient specimen, biotinylated DHEA-S antibodies, after addition of streptavidin-coated microparticles and a DHEA‑S derivative labeled with a ruthenium complex, the still‑vacant sites of the biotinylated antibodies become occupied, with formation of an antibody‑hapten complex. The entire complex becomes bound to the solid phase via interaction of biotin and streptavidin.The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier. Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve.
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Clinical information
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DHEA‑S is a steroid hormone for which the adrenal glands is the sole source in females and the principle source in males. Measurement of DHEA‑S can be useful in the diagnostic work‑up of female patients presenting with clinical symptoms of hyperandrogenism. Elevated DHEA‑S levels are indicative of an involvement of the adrenal gland. A decrease of DHEA‑S and total serum testosterone by more than 50 % upon dexamethasone suppression, is seen as confirmation of hyperandrogenism of the adrenal gland. (Roche Coba DHEA-S package insert ; Roche Diagnostic Corp., Indianapolis, IN
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Aliases
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DHEA-S, Dehydroepiandrosterone Sulfate
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
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Storage and transport instructions
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Storage: 5 days at 20 – 25°C
14 days at 2 – 8°C;
12 months at ‑20 °C (± 5 °C)
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample and heat‑inactivated samples.
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Biological reference intervals and clinical decision values
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Sex
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Age
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Reference interval (umol/L)
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From
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To
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Female
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9 years
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14 years
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0.92 – 7.60
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Female
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15 years
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19 years
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1.77 – 9.99
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Female
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20 years
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24 years
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4.02 - 11.0
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Female
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25 years
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34 years
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2.68 - 9.23
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Female
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35 years
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44* years
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1.65 - 9.15
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Female
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45 years
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54* years
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0.96 - 6.95
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Female
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55 years
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64 years
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0.51 - 5.56
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Female
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65 years
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74 years
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0.26 - 6.68
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Female
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≥ 75 years
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0.33 - 4.18
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Male
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9 years
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14 years
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0.66 - 6.70
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Male
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15 years
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19 years
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1.91 - 13.4
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Male
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20 years
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24 years
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5.73 - 13.4
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Male
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25 years
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34 years
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4.34 - 12.2
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Male
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35 years
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44 years
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2.41 - 11.6
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Male
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45 years
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54 years
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1.20 - 8.98
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Male
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55 years
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64 years
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1.40 - 8.01
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Male
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65 years
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74 years
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0.91 - 6.76
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Male
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≥ 75
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0.44 - 3.34
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Female/Male
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< 1 week
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2.93 - 16.5
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Female/Male
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1week
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4 weeks
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0.86 - 11.7
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Female/Male
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1 month
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12 months
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0.09 - 3.35
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Female/Male
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1 year
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4 years
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0.01 - 0.53
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Female/Male
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5 years
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9 years
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0.08 - 2.31
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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