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Test ID: LH
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Luteinizing Hormone
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Useful For
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To evaluate fertility issues and reproductive organs and pituitary function.
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Method name and description
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Electrochemiluminescence Immunoassay (ECLIA)
Electrochemiluminescence immunoassay (ECLIA) intended for use on the cobas e 801 immunoassay analyzer. Patient specimen, a biotinylated monoclonal LH‑specific antibody, and a monoclonal LH‑specific antibody labeled with a ruthenium complex form a sandwich complex. After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve.
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Clinical information
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FSH (follicle stimulating hormone), together with LH (luteinizing hormone), belongs to the gonadotropin family. FSH and LH regulate and stimulate the growth and function of the gonads (ovaries and testes) synergistically. In women, the gonadotropins act within the hypothalamus‑pituitary‑ovary regulating circuit to control the menstrual cycle. Determination of the LH concentration is used in the elucidation ofdysfunctions within the hypothalamus‑pituitary‑gonads system. TThe determination of LH in conjunction with FSH is utilized for the following indications: congenital diseases with chromosome aberrations (e.g. Turner's syndrome), polycystic ovaries (PCO), clarifying the causes of amenorrhea, menopausal syndrome, and suspected Leydig cell insufficiency.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
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Storage and transport instructions
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Storage: 5 days at 20 – 25°C
14 days at 2 – 8°C;
6 months at ‑20 °C (± 5 °C)
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample and heat‑inactivated samples.
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Biological reference intervals and clinical decision values
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Female
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Reference Interval (IU/L)
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14 days
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3 years
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0.3 – 2.5
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3
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6 years
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0 -1.9
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6
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8 years
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0 - 3.0
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8
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10 years
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0 – 4.0
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10
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11 years
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0 - 6.5
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11
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12 years
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0.4 – 9.9
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12
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13 years
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0.3 – 5.4
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13
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14 years
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0.5 – 31.2
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14
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15 years
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0.5 – 20.7
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15
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16 years
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0.4 – 29.4
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16
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17 years
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1.6 -12.4
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Adult Female
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Follicular Phase
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2 – 13
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Ovulation Phase
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14 - 96
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Luteal Phase
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1 - 11
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Post- menopause
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8 - 59
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Male
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14 days
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10 years
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0.3 – 2.8
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10
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11 years
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0.3 -1.8
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11
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12 years
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0.3 – 4.0
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12
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13 years
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0.6 -6.0
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13
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14 years
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0.5 – 7.9
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14
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16 years
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0.5 -10.8
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16
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17 years
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0.9 – 5.9
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Adult Male
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1.7 – 8.6
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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