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Test ID: Salicylate
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Salicylate Level
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Useful For
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To assess and evaluate Salicylate toxicity in blood.
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Method name and description
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Photometric assay
Photometric assay performed on the Roche cobas c-systems. This determination depends upon the conversion of salicylate in the presence of NADH by salicylate hydroxylase to catechol and NAD. The concomitant conversion of NADH to NAD is measured by the decrease in absorbance at 340 nm. The decrease is proportional to the concentration of salicylate present in the sample.
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Clinical information
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Salicylate is a common drug used in many formulations due to its analgesic and anti‑inflammatory properties. Salicylate overdose can cause metabolic acidosis with a high anionic gap, gastrointestinal and central nervous system disturbances, as well as encephalopathy and renal failure. Therefore, a method for the rapid and accurate determination of salicylate is needed.
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Aliases
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Salicylate, Aspirin, Acetylsalicylic Acid (ASA)
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
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Storage and transport instructions
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Storage: 2 weeks at 4°C
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample.
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Biological reference intervals and clinical decision values
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Interpretative Data:
Therapeutic Ranges for anti‑inflammatory/rheumatic conditions: 15 -30 mg/dL
Toxic: >30 mg/dL
Lethal: >60 mg/dL
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
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