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Test ID: SHBG
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Sex Hormone Binding Globulin
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Useful For
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Evaluation of low testosterone (androgen deficiency) in men and symptoms of excess male hormones (androgens) in women.
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Method name and description
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Electrochemiluminescence immunoassay (ECLIA): Sandwich principle
Electrochemiluminescence immunoassay (ECLIA) intended for use on the cobas e 801 immunoassay analyzer. Patient specimen, biotinylated monoclonal SHBG‑specific antibodies, and a monoclonal SHBG‑specific antibody labeled with a ruthenium complex react to form a sandwich complex. After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier. Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve.
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Clinical information
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Sex hormone‑binding globulin (SHBG) is a blood transport protein for testosterone and estradiol (E2) produced mainly by the liver and its synthesis and secretion are regulated by estrogen and negatively influenced by liver fat content and inflammatory cytokines. Decreased SHBG serum levels are associated with conditions where elevated androgen levels are present or where the effect of androgen on its target organs is excessive. This explains the gender‑related differences seen between men and women, especially during puberty. Elevated SHBG levels can be seen in elderly men, and are often found in patients with hyperthyroidism and cirrhosis. SHBG levels also increase when oral contraceptives or antiepileptic drugs are taken. Decreased SHBG concentrations are often seen inpatients with hypothyroidism, polycystic ovarian syndrome, obesity, hirsutism, elevated androgen levels, alopecia, and acromegaly. (Roche Cobas SHBG package insert ; Roche Diagnostic Corp., Indianapolis, IN).
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Aliases
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Sex-Hormone Binding Globulin , SHBG
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Storage and transport instructions
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Storage: 5 days at 20 – 25°C
7 days at 2 – 8°C;
12 months at ‑20 °C (± 5 °C)
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample and heat‑inactivated samples.
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Biological reference intervals and clinical decision values
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Sex
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Age
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Reference interval (nmol/L)
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From
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To
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Male
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1 day
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30 days
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11 - 71
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Male
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31 days
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365 days
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60 - 209
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Male
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1 year
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3 years
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42 - 156
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Male
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3 years
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6 years
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39 -146
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Male
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6 years
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9 years
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38 -114
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Male
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9 years
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12 years
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32 - 93
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Male
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12 years
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15 years
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13 - 63
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Male
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15 years
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18 years
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11 – 54
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Male
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18 years
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49 years
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18.3 – 54.1
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Male
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49 years
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150 years
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20.6 -76.7
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Female
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1 day
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30 days
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12 - 51
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Female
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31 days
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365 days
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50 - 181
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Female
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1 year
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3 years
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51 - 158
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Female
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3 years
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6 years
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48 - 142
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Female
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6 years
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9 years
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31 - 103
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Female
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9 years
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12 years
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20 - 100
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Female
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12 years
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15 years
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17 - 77
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Female
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15 years
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18 years
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9 - 75
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Female
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18 years
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49 years
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32.4 - 128
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Female
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49 years
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150 years
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27.1 - 128
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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