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Test ID: IH WT 1
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Wilms Tumor
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Immunohistochemistry Stain
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Useful For
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Identification of Wilms tumor, acute leukemias, malignant mesothelioma, serous ovarian adenocarcinoma, granulosa cell tumor, and other tumors.
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Method name and description
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- Monoclonal Mouse Anti-Human Wilms’ Tumor 1 (WT1) Protein Clone 6F-H2
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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WT1 is a gene involved in the induction of Wilms’ tumor, a pediatric renal malignancy. The WT1 gene, located on chromosome 11p13, is inactivated in 5 to 10% of sporadic Wilms’ tumors and nearly 100% of Denys-Drash patients, a syndrome associated with genitourinary abnormalities and Wilms’ tumor. WT1 encodes a zinc finger transcription factor which recognizes the early growth response (EGR-1) consensus sequence found in promoters of growth factor genes. The protein encoded by WT1 regulates transcription of other genes and can function both as a transcriptional activator and repressor. WT1 has been demonstrated to repress transcription of various growth-related genes such as platelet-derived growth factor A chain (PDGF-A) and insulin-like growth factor (IGF). The transcriptional activity of this protein, however, can be modulated through physical interactions between WT1 and p53. In the absence of wild type p53, WT1 has been demonstrated to function as a transcriptional activator of the EGR-1 promoter in transfection assays.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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