|
Test ID: Thiamine (vitamin B1, whole blood)
|
|
Thiamine (vitamin B1, whole blood)
|
|
Thiamine (vitamin B1, whole blood)
|
|
Useful For
|
Assessment of thiamine (vitamin B1) status
|
|
Method name and description
|
High performance liquid chromatography (HPLC) with fluorescence detection.
|
|
Clinical information
|
Thiamine (vitamin B1) is a water-soluble vitamin with functions in the nervous sytem, musculoskeletal system, and in energy metabolism. A deficiency in this enzyme can therefore lead to signs and symptoms related to these functions. Chronic thiamine deficiency can result in beri-beri with neurological symptoms (dry beri-beri), or cardiovascular effects (wet beri-beri).
|
|
Aliases
|
Thiamine pyrophosphate (TPP)
Vitamin B1
|
|
|
Specimen type / Specimen volume / Specimen container
|
Specimen type: whole blood.
Specimen volume: 2 - 5 mL of whole blood sample and must be protected againest light.
Specimen container: EDTA collection tube.
|
|
Collection instructions / Special Precautions / Timing of collection
|
Whole blood sample must be protected against light.
|
|
Relevant clinical information to be provided
|
Presenting clinical signs and symptoms
|
|
Storage and transport instructions
|
Freshly collected blood samples must be protected from light and transported to the laboratory as soon as possible.
Blood samples are stable for up to 5 hours when stored at room temperature (15-30°C) and protected from light.
Refrigerated samples (2-8°C) that are protected from light are stable for up to 24 hours.
Frozen samples (-18°C) that are protected from light are stable for up to 28 days.
Samples should be transported according to their storage conditions
|
|
Specimen Rejection Criteria
|
Insufficient specimen quantity.
Specimen is not protected against light.
Wrong collection container.
Clotted sample.
|
|
|
Biological reference intervals and clinical decision values
|
|
|
|
Factors affecting test performance and result interpretation
|
Samples not protected from light may lead to misleading results.
|
|
Turnaround time / Days and times test performed / Specimen retention time
|
Turnaround time: 5 working days.
Days and times test performed: once a week.
Specimen retention time: 48 hours.
|
|
|
