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Test ID: IH Stat6
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Signal Transducer and Activator of Transcription 6
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Immunohistochemistry Stain
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Useful For
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Distinguishing solitary fibrous tumors from other morphologically similar tumors.
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Method name and description
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- Rabbit Monoclonal Antibody STAT6 (EP325)
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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STAT6, a member of the signal transducers and activators of transcription (STAT) family, has been found to form recurrent fusions with NAB2 on chromosome 12q13 in most solitary fibrous tumors. Inactivated STAT6 can be found in the form of a dimer located in the cytoplasm. STAT6 and NAB2 fusion enables cytosolic STAT6 to migrate to the nucleus and thus allowing for detection in immunohistochemical assays. NAB2-STAT6 fusion transcriptions have been reported in the majority of solitary fibrous tumors but not in meningiomas, hemangioblastomas, schwannomas, and hemangiomas.
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Aliases
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Signal Transducer and Activator of Transcription 6.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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