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Test ID: Voriconazole
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Voriconazole
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Useful For
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Monitoring levels of Voriconazole in conjunction with clinical evaluations to ensure appropriate therapy.
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Method name and description
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ARK Voriconazole Assay is a homogeneous immunoassay based on competition between drug in the specimen and voriconazole labeled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH) for binding to the antibody reagent. As the latter binds antibody, enzyme activity decreases. In the presence of drug from the specimen, enzyme activity increases and is directly proportional to the drug concentration. Active enzyme converts the coenzyme nicotinamide adenine dinucleotide (NAD) to NADH that is measured spectrophotometrically as a rate of change in absorbance. Endogenous serum G6PDH does not interfere with the results because the coenyzme NAD functions only with the bacterial enzyme used in the assay.
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Clinical information
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Voriconazole is a triazole antifungal agent indicated for use in the treatment of invasive aspergillosis, candidemia, esophageal candidiasis and serious infections caused by Scedosporium apiospermum and Fusarium species including Fusarium solani, in patients intolerant of, or refractory to, other therapy.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum ONLY
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
Time of blood draw since last dose should be noted. Trough sample is generally acceoted.
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Storage and transport instructions
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Storage: 1 week at 2 – 8°C;
4 weeks at ‑20 °C or less
Transport: 2-8°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample.
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Biological reference intervals and clinical decision values
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Therapeutic range in adult patients: > 1 to 5 mcg/mL
Toxicity: > 5.5 mcg/mL
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
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