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Test ID: IH Pan TRK
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Neurotrophic tyrosine receptor kinase
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Immunohistochemistry Stain
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Useful For
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NTRK fusions are characteristic of a few rare types of cancer, such as secretory carcinoma of the breast or salivary gland and infantile fibrosarcoma, but they are also infrequently seen in some common cancers, such as melanoma, glioma and carcinomas of the thyroid, lung and colon.
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Method name and description
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Rabbit monoclonal Pan TRK (RM423)
Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section.
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Clinical information
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Neurotrophic tyrosine receptor kinase (NTRK) proto-oncogenes NTRK1, NTRK2, and NTRK3 (that encode TRK A, TRK B, and TRK C proteins, respectively) may form gene fusions through their kinase domains, driving tumor development . TRK A is activated by nerve growth factor (NGF), TRK B by brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4), and TRK C by neurotrophin3 (NT-3).
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Aliases
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Neurotrophic tyrosine receptor kinase (NTRK)
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Specimen type / Specimen volume / Specimen container
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Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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Broken slides
Unlabeled slides with patient/case identification
Contaminated slides
Slides/paraffin blocks mismatch
Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day
- Shelf-life of the paraffin section slides: ≤6 weeks
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