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Test ID: IH FOXL2
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Forkhead box L2
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Immunohistochemistry Stain
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Useful For
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FOXL2 antibody is a relatively sensitive and highly specific marker for Sex Cord-Stromal Tumors (SCST). FOXL2 antibody expression is present in almost all SCSTs with a FOXL2 mutation or without a mutation. A specific somatic mutation in the FOXL2 gene has been found in Adult Granulosa Cell Tumor (402C→G / C134W), which is present in 70-95% of Ovarian Adult Granulosa Cell Tumors but not in Ovarian Fibromas.
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Method name and description
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Rabbit Polyclonal (RPab)
Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section.
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Clinical information
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The Forkhead box L2 (FOXL2) gene encodes FOXL2, a transcription factor involved in ovarian development and function. Before birth and in adulthood, the FOXL2 protein regulates the growth and proliferation of hormone-producing ovarian granulosa cells. FOXL2 is also involved in the breakdown of fats, steroid hormones, and potentially harmful reactive oxygen species in the ovaries. A specific somatic mutation in the FOXL2 gene has been found in Adult Granulosa Cell Tumor (402C→G / C134W), which is present in 70-95% of Ovarian Adult Granulosa Cell Tumors but not in Ovarian Fibromas. This mutation is also present in 2 of 5 men with Adult Granulosa Cell Tumor but absent in Ovarian Juvenile Granulosa Cell Tumors.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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Broken slides
Unlabeled slides with patient/case identification
Contaminated slides
Slides/paraffin blocks mismatch
Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day
- Shelf-life of the paraffin section slides: ≤6 weeks
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