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Test ID: IH TIA-1
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T-cell intracytoplasmic antigen
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Immunohistochemistry Stain
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Useful For
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TIA-1 antibody reacts in anaplastic large cell lymphomas (ALCL), NK-cell lymphomas, peripheral T-cell lymphomas, T-cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, and Hodgkin’s lymphoma. TIA-1 also reacts with most large granular lymphocytic leukemias, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas and pulmonary angiocentric lymphomas of T-or NK-phenotype. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias are negative for TIA-1 antibody.
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Method name and description
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Rabbit Monoclonal TIA-1(RBT-TIA1)
Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section.
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Clinical information
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TIA-1 (T-cell intracytoplasmic antigen) is a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. TIA-1 is a member of an RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15 kDa protein thought to be derived from the carboxyl terminus of the 40 kDa product by proteolytic processing.
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Aliases
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T-cell intracytoplasmic antigen (TIA-1)
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Specimen type / Specimen volume / Specimen container
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Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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Broken slides
Unlabeled slides with patient/case identification
Contaminated slides
Slides/paraffin blocks mismatch
Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day
- Shelf-life of the paraffin section slides: ≤6 weeks
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