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Test ID: IH ROS1
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ROS1
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Immunohistochemistry Stain
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Useful For
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The identification of ROS1 rearrangement could be helpful in characterizing non-small cell lung carcinoma.
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Method name and description
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Rabbit Monoclonal ROS1 (MRQ-68)
Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section.
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Clinical information
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ROS1 (MRQ-68) is an antibody designed to target the c-terminal region of the ROS1 protein. ROS1 encodes a membranous protein with a single transmembrane domain comprising an intracellular-facing receptor tyrosine kinase at the c-terminal region, and an extracellular N-terminal domain comprising fibronectin-like repeats. ROS1 is believed to be expressed during development; however, its normal purpose and functionality have not been well-defined. Upregulated ROS1 expression occurs in lesions as a result of chromosomal rearrangement of ROS1 attached to one of several possible characterized gene partners which are more constitutively expressed. Of the characterized gene rearrangements that can occur, the receptor tyrosine kinase region is usually conserved when paired with an upstream gene partner. Visualization of ROS1 is dependent upon the upstream partner gene, and can be observed in the plasma membrane, cytosol, or Golgi Apparatus
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Specimen type / Specimen volume / Specimen container
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Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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Broken slides
Unlabeled slides with patient/case identification
Contaminated slides
Slides/paraffin blocks mismatch
Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day
- .Shelf-life of the paraffin section slides: ≤6 weeks
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